Alternative Names:

Vinculin;VCL;Metavinculin; MV;

Application:

WB ICC/IF IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:50

Purification:

Affinity-chromatography

Specificity:

Vinculin Antibody detects endogenous levels of total Vinculin

Immunogen:

A synthesized peptide derived from human Vinculin

Description:

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail. In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.