Alternative Names:

CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; IF4E; MGC111573; mRNA cap-binding protein

Application:

WB  IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000    IHC 1:50-1:200

Western blot analysis of eIF4E phosphorylation expression in FBS treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at Serine 209

Immunogen:

A synthesized peptide derived from human eIF4E around the phosphorylation site of Serine 209

Description:

eIF4E a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.