Alternative Names:

Aminoglycoside 3' phosphotransferase; APH(3')II; aphA; Neomycine phosphotransferase; NPTII;

Application:

WB

Reactivity:

recombinant protein

Dilution:

WB 1:1000

Western blot detection of NPTⅡ in non-trangenic Brassica napus L. and transgenic Brassica napus L. cell lysates using NPTⅡ mouse mAb (1:1000 diluted).Predicted band size:29KDa.Observed band size:29KDa.

Purification:

Affinity-chromatography

Specificity:

NPTII Mouse mAb detects endogenous levels of total NPTII

Immunogen:

Description:

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.