产品介绍

• 抗原名称

  alpha smooth muscle Actin (SMA)

• 分子别名

  Alpha-actin-2,ACTA2

• 免疫原

  Synthetic Peptide

• Accession

  
  P62736

• 克隆号

  SDT-132-63

• 抗体类型

  Rabbit mAb

• 反应种属

  Hu,Ms,Rat

• 纯化方式

  Protein A

• 浓度

  2 mg/ml

• 性状

  Liquid

• 缓冲体系

  PBS, 0.1% BSA, 0.01% Proclin 300

• 储存条件

  12 months from date of receipt / reconstitution, 4 °C as supplied.

• • IF

    1:500

• α-smooth muscle actin, encoded by ACTA2, is expressed in abundance in vascular smooth muscle, comprising 50–70% of total actin, with the remainder composed of β-cytoplasmic and γ-actins. Whereas Sα-smooth muscle expression is normally restricted to smooth muscle cells, it can also be expressed in nonmuscle cells, most notably myofibroblasts that use cell traction forces to remodel extracellular matrix.
  

• 免疫细胞化学

  • 

    IF shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-alpha smooth muscle Actin (Alexa Fluor? 488 Conjugate) antibody was used at 1/500 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 

    IF shows positive staining in paraffin-embedded human colon. Anti-alpha smooth muscle Actin (Alexa Fluor? 488 Conjugate) antibody was used at 1/500 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 

    IF shows positive staining in paraffin-embedded mouse stomach. Anti-alpha smooth muscle Actin (Alexa Fluor? 488 Conjugate) antibody was used at 1/500 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

  • 

    IF shows positive staining in paraffin-embedded rat stomach. Anti-alpha smooth muscle Actin (Alexa Fluor? 488 Conjugate) antibody was used at 1/500 dilution (Green). Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.