产品介绍

• 宿主来源

  Rabbit

• 分子别名

  Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8 (MRP-8; p8), S100 calcium-binding protein A8, Urinary stone protein band A, CAGA

• 细胞定位

  Cytoplasm,Cell membrane,Secreted

• Accession

  
  P05109

• 克隆号

  S-R251

• 抗体类型

  Recombinant mAb

• 应用

  IHC-P,WB

• 反应种属

  Hu,Ms,Rat

• 纯化方式

  Protein A

• 浓度

  0.5 mg/ml

• 标记

  Unconjugated

• 性状

  Liquid

• 缓冲体系

  PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

• 储存条件

  12 months from date of receipt / reconstitution, -20 °C as supplied

• • WB

    1:1000

  • IHC

    1:2000

• S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as calgranulin A. It is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis and post COVID-19 condition.

• 免疫印迹

  • 

    WB result of S100A8/MRP8 Rabbit mAb
    Primary antibody: S100A8/MRP8 Rabbit mAb at 1/1000 dilution
    Lane 1: mouse spleen lysate 20 μg
    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 10 kDa
    Observed MW: 10 kDa
    Exposure time: 180 s

  • 

    WB result of S100A8/MRP8 Rabbit mAb
    Primary antibody: S100A8/MRP8 Rabbit mAb at 1/1000 dilution
    Lane 1: rat spleen lysate 20 μg
    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
    Predicted MW: 10 kDa
    Observed MW: 10 kDa
    Exposure time: 60 s (This blot was developed with high sensitivity substrate)

• 免疫组化

  • 

    IHC shows positive staining in paraffin-embedded human spleen. Anti-S100A8/MRP8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human tonsil. Anti-S100A8/MRP8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human liver. Anti-S100A8/MRP8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human breast cancer. Anti-S100A8/MRP8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-S100A8/MRP8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human Hodgkin’s lymphoma. Anti-S100A8/MRP8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded mouse spleen. Anti-S100A8/MRP8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded rat spleen. Anti-S100A8/MRP8 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.