新型冠状病毒检测引物探针
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- 2022-07-12 12:00:35
上海闪晶分子生物科技有限公司
闪晶生物按照WHO公布的用于检测新型冠状病毒（COVID-19)的基因N、E、RdRP合成了对应探针、引物和阳性对照质粒，内参基因的使用可以增加样品有效性的判断！
新冠病毒2019-nCoV/Sars-CoV-2, complete genome Sequence ID: MN997409.1 Length: 29882
| Name | Country | Label 5’ | Oligonucleotide Sequence (5’>3’) | Label 3’ | Position |
| N Protein | |||||
| 2019-nCoV_N1-F | USA | GACCCCAAAATCAGCGAAAT | 28287-28306 | ||
| 2019-nCoV_N1-R | TCTGGTTACTGCCAGTTGAATCTG | 28335-28358 | |||
| 2019-nCoV_N1-P | FAM | ACCCCGCATTACGTTTGGTGGACC | BQ1 | 28309--28332 | |
| 2019-nCoV_N2-F | TTACAAACATTGGCCGCAAA | 29164-29183 | |||
| 2019-nCoV_N2-R | GCGCGACATTCCGAAGAA | 29213-29230 | |||
| 2019-nCoV_N2-P | FAM | ACAATTTGCCCCCAGCGCTTCAG | BQ1 | 29188--29210 | |
| 2019-nCoV_N3-F | GGGAGCCTTGAATACACCAAAA | 28681-28702 | |||
| 2019-nCoV_N3-R | TGTAGCACGATTGCAGCATTG | 28732-28752 | |||
| 2019-nCoV_N3-P | FAM | AYCACATTGGCACCCGCAATCCTG | BQ1 | 28706-28727 | |
| 2019-nCoV-NF | China | GGGGAACTTCTCCTGCTAGAAT | 28881-28902 | ||
| 2019-nCoV-NR | CAGACATTTTGCTCTCAAGCTG | 28958-28979 | |||
| 2019-nCoV-NP | FAM | TTGCTGCTGCTTGACAGATT | TAMRA | 28934-28953 | |
| HKU-NF | Hong Kong | TAATCAGACAAGGAACTGATTA | 29145-29166 | ||
| HKU-NR | CGAAGGTGTGACTTCCATG | 29236-29254 | |||
| HKU-NP | FAM | GCAAATTGTGCAATTTGCGG | TAMRA | 29196-29177 | |
| NIID_2019-nCOV_N_F2 | Japanese | AAATTTTGGGGACCAGGAAC | 29142-29161 | ||
| NIID_2019-nCOV_N_R2 | TGGCAGCTGTGTAGGTCAAC | 29280-29299 | |||
| NIID_2019-nCOV_N_P2 | FAM | ATGTCGCGCATTGGCATGGA | BQ1 | 29239-29258 | |
| WH-NIC N-F | Thailand | CGTTTGGTGGACCCTCAGAT | 28320-28339 | ||
| WH-NIC N-R | CCCCACTGCGTTCTCCATT | 28358-28376 | |||
| WH-NIC N-P | FAM | CAACTGGCAGTAACCA | MGB | 28341-38356 | |
| E gene | |||||
| E_Sarbeco_F1 | Germany | ACAGGTACGTTAATAGTTAATAGCGT | 26269-26294 | ||
| E_Sarbeco_R2 | ATATTGCAGCAGTACGCACACA | 26360-26381 | |||
| E_Sarbeco_P1 | FAM | ACACTAGCCATCCTTACTGCGCTTCG | BQ1 | 26332-26357 | |
| CoV-EFP | China | ACTTCTTTTTCTTGCTTTCGTGGT | 26295-26318 | ||
| CoV-ERP | GCAGCAGTACGCACACAATC | 26357-26376 | |||
| CoV-EP | FAM | CTAGTTACACTAGCCATCCTTACTGC | BQ1 | 26326-26351 | |
| ORF1ab | |||||
| 2019-nCoV-OFP | China | CCCTGTGGGTTTTACACTTAA | 13342-13362 | ||
| 2019-nCoV-ORP | ACGATTGTGCATCAGCTGA | 13442-13460 | |||
| 2019-nCoV-OP | FAM | CCGTCTGCGGTATGTGGAAAGGTTATGG | BQ1 | 13377--13404 | |
| HKU-ORF1b-nsp14F | Hong Kong | TGGGGYTTTACRGGTAACCT | 18778-18797 | ||
| HKU- ORF1b-nsp14R | AACRCGCTTAACAAAGCACTC | 18889-18909 | |||
| HKU-ORF1b-nsp141P | FAM | TAGTTGTGATGCWATCATGACTAG | TAMRA | 18849--18872 | |
| RdRP_SARSr-F2 | Germany | GTGARATGGTCATGTGTGGCGG | 15431-15452 | ||
| RdRP_SARSr-R1 | CARATGTTAAASACACTATTAGCATA | 15435-15460 | |||
| RdRP_SARSr-P2 | FAM | CAGGTGGAACCTCATCAGGAGATGC | BQ1 | 15470-15494 | |
| Name | Label 5’ | Oligonucleotide Sequence (5’>3’) | Label 3’ | ||
| Internal standard | RNAse Protein/NM_006413.5 | ||||
| RP-F | Recognized | AGATTTGGACCTGCGAGCG | 28-46 | ||
| RP-R | GAGCGGCTGTCTCCACAAGT | 73-92 | |||
| RP-P RNAse P | FAM | TTCTGACCTGAAGGCTCTGCGCG | BQ1 | 49-71 |
闪晶同时提供对应的阳性对应质粒或假RNA病毒！
相关参考文献：
1.(2020) 2019 Novel Coronavirus (2019-nCoV), Wuhan, China. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
2.(2020) 2019-Novel coronavirus (2019-nCoV) real-time rRT-PCR panel primers and probes. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
3.(2020) Real-time RT-PCR panel for detection 2019-novel coronavirus. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
4.(2020) Emergency use authorization. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
专业设计合成标记LNA荧光探针/分子信标探针/环状RNA探针/寨卡病毒荧光定量PCR探针/非编码RNA探针/ncRNA探针/lncRNA探针/circRNA探针/环状RNA探针/MGB探针/Taqman探针/exo探针
