背景介绍(Background)：

This protein is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]

 

Interleukin (IL)-15 is a 14-15 kDa four-helix bundle cytokine. IL-15, unlike other cytokines, is widely expressed by many cell types including monocytes, macrophages, dendritic cells (DCs), fibroblasts, epithelial cells, and skeletal muscle cells, but surprisingly not by T cells. It acts as a growth factor and also promotes the survival of T, B, and NK cells by preventing apoptosis through the upregulation of anti-apoptotic and downregulation of pro-apoptotic factors. IL-15 plays a crucial role in the development, homeostasis, and function of T, NK, and NK-T cells, and is additionally required for various functions of the B cells, DCs, macrophages, and mast cells. IL-15 is minimally secreted but is effectively delivered by trans-presentation in association with its unique receptor alpha (IL-15 Rα) on the surface of IL-15 producing cells to interact with a receptor complex composed of the IL-2R β and common γ chains on target cells. Trans-presented IL-15/IL-15 Rα signals through β and γ chains expressed on responding cells, leading to the recruitment and activation of JAK1 and JAK3. Activated JAK1 and JAK3 further phosphorylate STAT3 and STAT5, which prompts the transcription of IL-15-modulated genes in effector cells.

 

 

 

图 IL-15的信号转导

 

 

 

 

制剂(Formulation)：

Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4. 

 

质量控制(Quality Control)：

Purity: ≥ 95% as determined by reducing SDS-PAGE.    

Endotoxin: ＜ 0.1 EU/ug  as determined by LAL test.

 

保存(Storage)：

Use a manual defrost freezer and avoid repeated freeze - thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
6 months, -20 to -70℃ under sterile conditions after reconstitution.    

 

FOR RESEARCH USE ONLY

 

 

 

 

 

 

 

 

参考文献：

[1] Guo Y, et al. 2017. Cytokine Growth Factor Rev. 38:10-21.

[2] Patidar M, et al. 2016. Cytokine Growth Factor Rev. 31:49-59.