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IDH2(R172G) 小鼠单抗/2022动态已更新/武汉费斯德生物
Anti-IDH2(R172G) Mouse Monoclonal Antibody
  • 26321
  • NewEast Biosciences
  • 湖北省武汉市
  • 现货
  • 100ul
  • 4800
  • 2022-11-25 14:44:47

武汉费斯德生物科技有限公司

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  • 英文名称
  • Anti-IDH2(R172G) Mouse Monoclonal Antibody
  • 应用
  • ELISA, WB, IF, IHC
货号:   26231
基因符号:   IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH

描述:   Anti-IDH2(R172G) Mouse Monoclonal Antibody

背景介绍:   Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172G IDH2 mutations do exist in diffusely infiltrative gliomas.
免疫原:   A synthetic peptide from the internal region of IDH2 which includes the mutation of R172G, human origin
经过测试的应用:   ELISA, WB, IF, IHC
推荐稀释比:   
ELISA: 1: 1000-1: 5000
WB: 1: 100-1: 1000
IF: 1: 50-1: 100
IHC: 1: 50-1: 100
浓度:   1 mg/ml
Host:   Mouse
形式:   Liquid
克隆性:   Monoclonal
亚型: IgG  
纯度:   Purified from ascites
防腐剂: No
成分:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
种属反应性:   Recognizes R172G mutant, but not wild-type IDH2 of vertebrates
储存条件:    Store at -20°C. Avoid repeated freezing and thawing

蛋白质印迹:   

Western blot analysis of recombinant IDH2(R172G) and wildtype proteins.

Purified His-tagged IDH2(R172G) (lane 1) and corresponding wild-type IDH2 protein (lane 2) were blotted with Anti-IDH2(R172G) monoclonal antibody (Cat. #26231).

免疫荧光:   

Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172G) antibody.

HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172G) plasmid (right column), then fixed and stained with Anti-IDH2(R172G) monoclonal antibody (Cat. #26231).

 

品牌介绍:

  • NewEast Biosciences十二年前率先研发俩种独特的抗体,这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene
  • 我们的产品被将近一千篇同行评议的文章所引用。
  • 与国内外知名科研院校、三甲医院、生物公司等建立过稳定、持续的合作。
  • 致力于为全球科研用户提供高质量的进口生物产品,及一站式的科学技术服

 

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