IDH2(R172G) 小鼠单抗/2022动态已更新/武汉费斯德生物
Anti-IDH2(R172G) Mouse Monoclonal Antibody
- 26321
- NewEast Biosciences
- 湖北省武汉市
- 现货
- 100ul
- 4800 元
- 2022-11-25 14:44:47
武汉费斯德生物科技有限公司
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- 英文名称
- Anti-IDH2(R172G) Mouse Monoclonal Antibody
- 应用
- ELISA, WB, IF, IHC
货号: 26231 |
基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
描述: Anti-IDH2(R172G) Mouse Monoclonal Antibody |
背景介绍: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172G IDH2 mutations do exist in diffusely infiltrative gliomas. |
免疫原: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172G, human origin |
经过测试的应用: ELISA, WB, IF, IHC |
推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IF: 1: 50-1: 100 IHC: 1: 50-1: 100 |
浓度: 1 mg/ml |
Host: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG |
纯度: Purified from ascites |
防腐剂: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Recognizes R172G mutant, but not wild-type IDH2 of vertebrates |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
Western blot analysis of recombinant IDH2(R172G) and wildtype proteins.
Purified His-tagged IDH2(R172G) (lane 1) and corresponding wild-type IDH2 protein (lane 2) were blotted with Anti-IDH2(R172G) monoclonal antibody (Cat. #26231).
免疫荧光:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172G) antibody.
HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R172G) plasmid (right column), then fixed and stained with Anti-IDH2(R172G) monoclonal antibody (Cat. #26231).
品牌介绍:
- NewEast Biosciences十二年前率先研发俩种独特的抗体,这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene
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