IDH2(R172K) 小鼠单抗#2022价格已更新《推荐》
- 26163
- NewEast Biosciences
- 湖北省武汉市
- 现货
- 100ul
- 4800 元
- 2022-11-25 11:01:47
武汉费斯德生物科技有限公司
- 英文名称
- Anti-IDH2(R172K) Mouse Monoclonal Antibody
- 关键词
- Anti-IDH2抗IDH2小鼠单抗R172K抗体武汉费斯德生物公司
- 应用
- ELISA, WB, IF, IHC
| 货号: 26163 |
| 基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
|
描述: Anti-IDH2(R172K) Mouse Monoclonal Antibody
|
|
背景介绍: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas. NewEast Biosciences 是开发突变Oncogene抗体的先驱, 并拥有用于Oncogene研究较完整的抗体系列。该系列仅与突变的Oncogene结合，而不与相应的野生型结合。这些抗体可以 用于研究突变的Oncogene在肿瘤组织中的分布。 |
| 免疫原: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin |
| 经过测试的应用: ELISA, WB, IHC |
| 推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IHC: 1: 50-1: 100 IHC: 1: 10-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: recognizes R172K mutant, but not wild-type IDH2 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
|
蛋白质印迹:
Western blot analysis of recombinant IDH2(R172K) and wildtype proteins. Purified His-tagged IDH2(R172K) (lane 2) and wildtype protein (lane 1) were blotted with Anti-IDH2(R172K) monoclonal antibody (Cat. #26163). 免疫荧光:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172K) antibody. HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP- IDH2 (WT) plasmid, pCDNA3-GFP- IDH2 (R172H) plasmid , pCDNA3-GFP- IDH2 (R172W) plasmid, pCDNA3-GFP- IDH2 (R172G) plasmid,pCDNA3-GFP- IDH2 (R172M) plasmid or pCDNA3-GFP- IDH2 (R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163).
我公司将向你提供以下三种抗体或者试剂盒: (1) 仅识别 GTP酶的活性构型的产品, 它可以让你能够量化GTP酶在细胞中的活性和分布。(2) 识别突变 Oncogene蛋白, 但不认识相应野生型的抗体。 (3) 对 cAMP 和 cGMP 具有超亲和力（无需乙酰化）ELISA检测试剂盒。这些产品被将近一千篇同行评议的文章所引用。 2021年武汉费斯德生物科技有限公司和NewEast Biosciences收购了武汉纽斯特生物技术有限公司所有的产品和细胞株。因此, 纽斯特不再拥有这些产品销售权。如果您在 2022 年 6 月 1 日之后从 纽斯特购买相同的产品, 你不应该在你的论文中引用 纽斯特的产品为NewEast Biosciences产品。 ** 本公司销售的所有产品仅供实验科研使用，不用于人体及临床诊断。 |
