骨形态发生蛋白2抗体
BMP2
- bs-1012R
- 北京博奥森
- 北京市
- 现货
- 按需
- 议价
- 2023-10-18 10:00:15
北京博奥森生物技术有限公司
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- 英文名称
- BMP2
- 别名
- Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A; BMP-2; BMP2_HUMAN.
| 研究领域 | 细胞生物 免疫学 发育生物学 神经生物学 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13/44kDa |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMP2: 283-365/396 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis. Function: Induces cartilage and bone formation. Subunit: Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN. Subcellular Location: Secreted. Tissue Specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. Similarity: Belongs to the TGF-beta family. SWISS: P12643 Gene ID: 650 Database links: Entrez Gene: 650 Human Entrez Gene: 12156 Mouse Omim: 112261 Human SwissProt: P12643 Human SwissProt: P21274 Mouse Unigene: 73853 Human Unigene: 103205 Mouse Unigene: 90931 Rat 神经干细胞标志物 BMP-2主要对未分化间充质细胞和骨系细胞起到募集和分化作用。在骨形成早期，BMP-2不仅可使未分化间质细胞向骨形成中心募集，并分化为骨系细胞，而且可使成纤维细胞、成肌细胞及骨髓的基细胞逆转分化为骨系细胞。其主要过程是：增加或抑制这些细胞内的某些特异性蛋白的分泌，使成纤维细胞分化为成骨细胞，成肌细胞快速分化为肥大的软骨细胞，并促进基质钙化。 对于成骨细胞，BMP-2则可使之维持其特有细胞表型，并诱导成骨细胞标志物的增高，促进细胞外基质钙化。在骨形成后期，BMP-2还作为一种破骨细胞分化因子与其它支持破骨细胞分化因子直接或间接刺激破骨细胞分化，参与骨的重建。 |
| 产品图片 |
 Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti- BMP2 (bs-1012R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 13 kD Observed band size: 11 kD  Sample:
Lane 1: U2os (Human) Cell Lysate at 30 ug Lane 2: MG63 (Human) Cell Lysate at 30 ug Lane 3: Huvec (Human) Cell Lysate at 30 ug Primary: Anti-BMP2 (bs-1012R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60/45 kD Observed band size: 60 kD  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP2) Polyclonal Antibody, Unconjugated (bs-1012R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
 Tissue/cell: rat tibia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated(bs-1012R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
