DMEM+15%FBS+0.1uL/mL LIF+1%L-谷氨酰胺+1%NEAA+1%丙酮酸钠+1%PS+1uL/mLβ－巯基乙醇+1%P/S 培养瓶用0.1%明胶包被细胞呈集落生长