酸性磷酸酶（acid phosphatase，ACP）主要存在于巨噬细胞，定位于溶酶体内。ACP测定主要用于前列腺癌的辅助诊断。Acid Phosphatase from potatoProduct Number: P9730Storage Temperature: -20 °CCAS Number: 9001-77-8EC Number: 3.1.3.2Synonyms: Apase; Orthophosphoric-monoester phosphohydrolase (acid optimum)Product DescriptionAcid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters andanhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7 by the following reaction: R-PO4 + H2O→R-OH + HOPO32+Their function in the production, transport, and recycling of phosphate is critical for the metabolic and energy transductionprocesses of the cell. As a group, APases may be as important as kinases in regulatory processes.Plant APases have been localized in the cytosol, vacuoles,and cell walls. One key role is phosphate acquisition to mobilizeorganic phosphates in the soil.Phosphate starvation also induces APase generation.Potato tuber APase is a monomeric glycoprotein. The carbohydrate component makes up 16.6% of the molecular mass.Molecular mass: 69 kDaCarbohydrate residues (%):Mannose 5.0, Glucosamine 3.6, Rhamnose 3.4, Glucose 2.5, Galactose 1.5pH Optimum: 5.0–5.3pH Range: 4–7Temperature optimum: 37 °CSubstrates:α-glyceryophosphate; ATP, fructose-6-phosphate, fructose-6-phosphate, inorganic pyrophosphate,glucose-6-phosphate, p-nitrophenyl phosphateKM(mM): p-nitrophenyl phosphate 1.25, inorganic pyrophosphate 40.0 Activators:Cu2+, Mg2+, Mn2+, Zn2+, Hg2+(below 0.4 mM) Inhibitors:Al3+, Hg2+(above 0.4 mM), MoO42–, Zn2+, ureaThis product is partially purified from potato tubers and is supplied as a lyophilized powder.Specific activity: 0.5–3.0 units/mg solidUnit definition: One unit will hydrolyze 1.0 µmole of p-nitrophenyl phosphate per minute at pH 4.8 at 37 °C.APase is assayed spectrophotometrically in a 1.1 ml reaction mixture containing 41 mM citrate buffer, pH 4.8 at 37 °C, 6.9mM p-nitrophenyl phosphate, and 0.015–0.025 unit APase.Preparation InstructionsAPase is soluble in cold water (0.15–0.25 unit/ml). Prepare solution immediately before use.Storage/StabilityStore the product at –20 °C. When stored at –20 °C, the enzyme retains activity for at least one year.APase can lose half its activity after 30 minutes at 60 °C and 100% of its activity after two hours at 70 °C.Precautions and DisclaimerFor Laboratory Use Only. Not for drug, household or other uses.Solarbio备注：产品信息可能会有优化升级。请以实际标签信息为准。备注：以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。These protocols are for reference only. Solarbio does not independently validate these tmethods.