Tissue/cell: rabbit carotid artery; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-8/CXCL8 Polyclonal Antibody, Unconjugated(bs-0780R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
白介素8抗体
IL-8/CXCL8 Rabbit pAb
- bs-0780R
- 北京博奥森
- 北京市
- 现货
- 按需
- 议价
- 2023-10-17 15:35:01
北京博奥森生物技术有限公司
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- 英文名称
- IL-8/CXCL8 Rabbit pAb
概述
产品编号
bs-0780R
产品分类
一抗
英文名称
IL-8/CXCL8 Rabbit pAb
中文名称
白介素8抗体
英文别名
Interleukin-8; Interleukin 8; Interleukin8;(Ala-IL-8)77; (Ser-IL-8)72; 3 10C; 310C; AMCF 1; AMCF1; b ENAP; Bbeta thromboglobulin like protein; Beta thromboglobulin like protein; C-X-C motif chemokine 8; CXC chemokine ligand 8; CXCL 8; CXCL8; Emoctakin; GCP 1; GCP-1; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; GCP1; Granulocyte chemotactic protein 1; IL 8; IL-8; IL-8(1-77); IL-8(9-77); IL8; IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; IL8_HUMAN; Inteleukin 8; Interleukin8; K 60; K60; LECT; LUCT; Lymphocyte derived neutrophil activating factor; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-b; MDNCF-c; MONAP; Monocyte derived neutrophil activating peptide; Monocyte derived neutrophil activating protein; Monocyte derived neutrophil chemotactic factor; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAF; NAP 1; NAP-1; NAP1; Neutrophil activating factor; Neutrophil activating peptide 1; Neutrophil activating protein 1; Neutrophil-activating factor; Neutrophil-activating protein 1; Protein 3 10C; Protein 3-10C; SCYB 8; SCYB8; Small inducible cytokine subfamily B member 8; T cell chemotactic factor; T-cell chemotactic factor; TSG 1; TSG1.
交叉反应
Human,Rabbit（predicted：Pig,Cow,Sheep）
抗体来源
Rabbit
免疫原
KLH conjugated synthetic peptide derived from human IL-8
亚型
IgG
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
8/9kDa
浓度
1mg/ml
储存液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
功能
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
亚基
Homodimer.
亚细胞定位
Secreted.
翻译后修饰
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
相似性
Belongs to the intercrine alpha (chemokine CxC) family.
数据库链接
背景资料
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
应用
| 应用 | 推荐稀释比例 |
|---|---|
| ELISA | 1:5000-10000 |
| IF | 1:100-500 |
| IHC-F | 1:100-500 |
| IHC-P | 1:100-500 |
图片资料
