Sample:
Lane 1: Human THP-1 cell lysates
Lane 2: Human U937 cell lysates
Primary: Anti-CCR-5 (bs-2514R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 43 kD
细胞表面趋化因子受体5抗体
CCR5 Rabbit pAb
- bs-2514R
- 北京博奥森
- 北京市
- 现货
- 按需
- 议价
- 2023-10-18 11:28:18
北京博奥森生物技术有限公司
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- 英文名称
- CCR5 Rabbit pAb
概述
产品编号
bs-2514R
产品分类
一抗
产品类型
质检1级
英文名称
CCR5 Rabbit pAb
中文名称
细胞表面趋化因子受体5抗体
英文别名
C-C chemokine receptor 5; CCR5; CC-CKR-5;CCR-5; CCCKR5; CD195; CD 195; CKR-5; CKR5; CMKBR5; FLJ78003; IDDM22; AM4-7; C C chemokine receptor type 5; C-C motif chemokine receptor 5 A159A; CC Chemokine Receptor Type 5; CCR5 chemokine (C C motif) receptor 5; Chemokine receptor CCR5; CHEMR13; HIV 1 Fusion Coreceptor; HIV1 fusion coreceptor; IDDM22; MIP-1 alpha receptor; CCR5_HUMAN.
交叉反应
Human,Mouse,Rat
抗体来源
Rabbit
免疫原
KLH conjugated synthetic peptide derived from human CCR-5
亚型
IgG
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
40kDa
浓度
1mg/ml
储存液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
功能
Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
亚基
Interacts with PRAF2. Interacts with HIV-1 surface protein gp120. Efficient ligand binding to CCL3/MIP-1alpha and CCL4/MIP-1beta requires sulfation, O-glycosylation and sialic acid modifications. Glycosylation on Ser-6 is required for efficient binding of CCL4. Interacts with ADRBK1. Interacts with ARRB1 and ARRB2. Interacts with human cytomegalovirus/HHV-5 protein UL78.
亚细胞定位
Cell membrane; Multi-pass membrane protein.
组织特异性
Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
相似性
Belongs to the G-protein coupled receptor 1 family.
数据库链接
Entrez Gene: 1234 Human
Entrez Gene: 12774 Mouse
Omim: 601373 Human
SwissProt: P51681 Human
SwissProt: P51682 Mouse
Unigene: 450802 Human
背景资料
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
应用
| 应用 | 推荐稀释比例 |
|---|---|
| ELISA | 1:5000-10000 |
| Flow-Cyt | 1μg/Test |
| IF | 1:100-500 |
| IHC-F | 1:100-500 |
| IHC-P | 1:100-500 |
| WB | 1:500-2000 |
图片资料
Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)) .
Primary Antibody:Rabbit Anti-CCR5 antibody(bs-2514R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Primary Antibody:Rabbit Anti-CCR5 antibody(bs-2514R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Tissue/cell: mouse intestinal tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCR5 Polyclonal Antibody, Unconjugated(bs-2514R-FITC) 1:100, overnight at 4°C; DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCR5 Polyclonal Antibody, Unconjugated(bs-2514R-FITC) 1:100, overnight at 4°C; DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
