Blank control (blue line): Mouse thymus cells (blue).
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
NK细胞受体2D（CD314）抗体
NKG2D Rabbit pAb
- bs-0938R
- 北京博奥森
- 北京市
- 现货
- 50ul
- 100ul
- 200ul
- 议价
- 2023-10-18 15:00:55
北京博奥森生物技术有限公司
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- 英文名称
- NKG2D Rabbit pAb
概述
产品编号
bs-0938R
产品分类
一抗
产品类型
质检1级
英文名称
NKG2D Rabbit pAb
中文名称
NK细胞受体2D（CD314）抗体
英文别名
NKG2-D type II integral membrane protein; KLRK1; natural killer cell group 2D; NKG2-D isoform b; Klrk1; Nkrp2; NKG2-D-activating NK receptor; NK cell receptor D; Killer cell lectin-like receptor subfamily K; NK lectin-like receptor; CD314; CD 314; CD314 antigen; D12S2489E; Killer cell lectin like receptor subfamily K member 1; Killer cell lectin-like receptor subfamily K member 1; KLR; Klrk1; NKG2 D activating NK receptor; NKG2 D type II integral membrane protein; NKG2-D-activating NK receptor; NKG2D_HUMAN; NKLLR; NKR P2; Nkrp2.
交叉反应
Human,Mouse（predicted：Rat）
抗体来源
Rabbit
免疫原
KLH conjugated synthetic peptide derived from rat NKG2D
亚型
IgG
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
25kDa
检测分子量
31-35 kDa
浓度
1mg/ml
储存液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
功能
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
亚基
Homodimer.
亚细胞定位
Membrane; Single-pass type II membrane protein.
组织特异性
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs).
相似性
Contains 1 C-type lectin domain.
数据库链接
Entrez Gene: 100528032 Human
Entrez Gene: 22914 Human
Entrez Gene: 27007 Mouse
Omim: 611817 Human
SwissProt: P26718 Human
SwissProt: O54709 Mouse
Unigene: 387787 Human
Unigene: 8217 Mouse
Unigene: 14544 Rat
背景资料
This locus represents naturally occurring read-through transcription between the neighboring KLRC4 (killer cell lectin-like receptor subfamily C, member 4) and KLRK1 (killer cell lectin-like receptor subfamily K, member 1) genes on chromosome 12. The read-through transcript includes an alternate 5' exon and lacks a significant portion of the KLRC4 coding sequence, including the start codon, and it thus encodes the KLRK1 protein. [provided by RefSeq, Dec 2010]
应用
| 应用 | 推荐稀释比例 |
|---|---|
| ELISA | 1:5000-10000 |
| Flow-Cyt | 2μg/Test |
| IF | 1:100-500 |
| IHC-P | 1:100-500 |
| WB | 1:500-2000 |
图片资料
Blank control:SW480.
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (bs-0938R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample: Lung (Mouse) Lysate at 40 ug
Primary: Anti- NKG2D (bs-0938R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 26 kD
Primary: Anti- NKG2D (bs-0938R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 26 kD
