Anti RAS(G12S) Mouse Monoclonal Antibody
Anti RAS(G12S) Mouse Monoclonal Antibody
- CR-M26186
- 科博瑞生物
- 湖北省武汉市
- 现货
- 100ul
- 4800 元
- 2024-06-11 16:25:16
科博瑞（武汉）生物科技有限公司
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- 英文名称
- Anti RAS(G12S) Mouse Monoclonal Antibody
RAS(G12S) [CR-M26186]
[100ul]
| Catalog Number: CR-M26186 |
| Gene Symbol: H-Ras; K-Ras; N-Ras |
|
Description: Anti RAS(G12S) Mouse Monoclonal Antibody
|
| Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G12S mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions. |
| Immunogen: A synthetic peptide from the internal region of RAS which includes the mutation of G12S, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes G12S mutant, but not wild-type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant RAS(G12S) and wildtype proteins. Purified His-tagged RAS(G12S) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti RAS(G12S) monoclonal antibody (Cat. #CR-M26186). |
| Immunofluorescence: |
 |
 |
| Immunofluorescence of cells expressing RAS proteins with Anti RAS(G12S) antibody. HEK293T cells were transfected with pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(WT)plasmid,pCDNA3-GFP Ras(G12S) plasmid, pCDNA3-GFP Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP Ras(G12R)plasmid or pCDNA3-GFP Ras(G12A)plasmid, then fixed and stained with Anti RAS(G12S) monoclonal antibody (Cat. #CR-M26186). |
| Publications: |
| 1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model Gene Therapy 21, 888-896 (October 2014) |
| 2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
