临床诊断/药物研发

化学发光法具有灵敏、准确、快速、样品用量少等优点，被广泛应用于临床检验和生命科学研究。特别是化学发光免疫技术已被应用于癌症、甲状腺疾病、心血管疾病、血液系统疾病、消化系统疾病等多种疾病的快速检验。此外，化学发光在药物成分分析及抗氧化性分析中均有重要应用，是药物研发的有力研究工具。

临床诊断方法必须具备稳定、准确的性质，并可灵敏检出痕量或超痕量目标物。BPCL超微弱发光测量仪灵敏度高、稳定性好，已被多家科研院校、医院或检测机构应用于临床诊断技术的开发研究中。同时BPCL超微弱发光测量仪可快速、灵敏、动态获取发光动力学曲线，这一特点对研究伴随着发光现象的生命过程及评价药物的抗氧化性至关重要。BPCL超微弱发光测量仪在临床诊断及药物研发应用中的例子有：

[1] Yang L L, Tao Y Z, Yue G Y, Li R B.; Qiu, B, Guo L H, Lin Z Y, Yang H H. Highly selective and sensitive electrochemiluminescence biosensor for p53 DNA sequence based on nicking endonuclease assisted target recycling and hyperbranched rolling circle amplification. Analytical chemistry, 2017, 88(10): 5097-5103.

[2]  Zhang S P, Zang L L, Zhang X Z, Dai H, Xu G, Zhang Q R, Yang C P, Lin Y Y. Signal-on

electrochemiluminescent immunosensor based on poly (amidoamine) dendrimer functionalized carbon nanodots amplification for ultrasensitive detection of α-fetoprotein. Electrochimica Acta, 2016, 196:67-74.

[3] Liu Y M, Mei L, Liu L J, Peng L F, Chen Y H, Ren S W. Sensitive chemiluminescence immunoassay by capillary electrophoresis with gold nanoparticles. Analytical Chemistry, 2011, 83(3):1137-1143.

[4] Zhang Q R, Dai H, Wang T, Li Y L, Zhang, S P, Xu G F, Chen S H, Lin Y Y. Ratiometric electrochemiluminescent immunoassay for tumor marker regulated by mesocrystals and biomimetic catalyst. Electrochimica Acta, 2016, 196: 565-571.

[5] He Y Z, Wang X X, Zhang Y Z, Gao F, Li Y X, Chen H Q, Wang L. An ultrasensitive chemiluminescent immunosensor for the detection of human leptin using hemin/G-quadruplex DNAzymes-assembled signal amplifier. Talanta, 2013, 116: 816-821.

[6] Shi G F, Cao J, Zhang J J, Huang K J, Liu Y M, Chen Y H, Ren S W. Aptasensor based on tripetalous cadmium sulfide-graphene electrochemiluminescence for the detection of carcinoembryonic antigen. Analyst, 2014, 139(22): 5827-5834.

[7] Ding X P, Wang X T, Chen L L, Qi J, Xu T, Yu B Y. Quality and antioxidant activity detection of Crataegus leaves using on-line high-performance liquid chromatography with diode array detector coupled to chemiluminescence detection. Food Chemistry, 2010, 120(3): 929-933.

[8] Wu L, Ding X P, Zhu D N, Yu B Y, Yan Y Q. Study on the radical scavengers in the traditional Chinese medicine formula Shengmai San by HPLC–DAD coupled with chemiluminescence (CL) and ESI–MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 2010, 52(4): 438-445.

[9] Huang X, Zhou Y H, Ma M H, Cai Z X, Li T. Chemiluminescence evaluation of antioxidant activity and prevention of DNA damage effect of peptides isolated from soluble eggshell membrane protein hydrolysate. Journal of Agricultural & Food Chemistry, 2010, 58(23):12137-12142.

[10] Zhou Z L, Yin W Q, Zhang H L, Feng Z C Xia J M. A new iridoid glycoside and potential MRB inhibitory activity of isolated compounds from the rhizomes of Cyperus rotundus L. Natural Product Research, 2013, 27(19): 1732-1736.

[11] Fu H F, Xie B J, Gang F, et al. Effect of esterification with fatty acid of β-cryptoxanthin on its thermal stability and antioxidant activity by chemiluminescence method. Food Chemistry, 2010, 122(3): 602-609.

[12] Huang X, Zhou Y H, Ma M H, Cai Z X, Li T. Chemiluminescence evaluation of antioxidant activity and prevention of DNA damage effect of peptides isolated from soluble eggshell membrane protein hydrolysate. Journal of Agricultural & Food Chemistry, 2010, 58(23): 12137-12142.