Methyl (mono) Arginine Antibody
- ICP0801
- ImmuneChem
- 广西壮族自治区南宁市
- 一周内
- 100ug支
- 2875 元
- 2022-08-03 17:21:29
南宁市蓝光生物技术有限公司
- 英文名称
- Methyl (mono) Arginine Antibody
Methyl (mono) Arginine Antibody
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Catalog# Product Description |
ICP0801 This antibody is raised from rabbits that were immunized with NG- mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are useful for detection, isolation and localization of proteins with Nγ-methylated arginine residues. |
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 Competitive ELISA: inhibition of the binding mMeR-HRP to anti-mMeR antibody immobilized (1µg/mL) by free mMeR (IC50=40-50 ng/mL)
Western blot of human MMRU cells with ICP0801 (A,B) plus competitive inhibition with free methyl arginine (C,D)
 Fluorescent Staining of MMRU cells |
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS, 50% glycerol
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Immunogen
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NG-mono-methyl arginine-KLH conjugates
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Purification
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Purified by antigen-specific affinity chromatography
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Specificity
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The antibody recognizes free mono-methyl arginine and proteins with methylated arginine residues. It does not cross react with either asymmetric or symmetric dimethyl arginine.
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Species Tested
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Pan-specific regardless of species.
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Applications
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ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
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Scientific Description
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Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is three years from date of shipping if properly stored.
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Product Specific References |
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