Screen Quest Fluo-8钙检测试剂盒是美国AAT Bioquest生产的钙检测试剂盒，钙通量测定是用于筛选G蛋白偶联受体（GPCR）的药物发现中的优选方法。 Screen Quest Fluo-8 NW钙测定试剂盒提供均匀的荧光测定，用于检测细胞内钙动员。表达感兴趣的GPCR的细胞通过钙预先加载我们专有的Fluo-8 NW，其可以穿过细胞膜。 Fluo-8 NW是可用于HTS筛选的亮钙指示剂。一旦进入细胞内，Fluo-8 NW的亲脂性阻断基团被非特异性细胞酯酶切割，产生带负电荷的荧光染料，其停留在细胞内，并且在与钙结合时其荧光大大增强。当用筛选化合物刺激细胞时，该受体表明细胞内钙的释放，这极大地增加了Fluo-8 NW的荧光。其长波长，高灵敏度和100-250倍荧光增加的特性（当它与钙形成复合物时）使Fluo-8 NW成为测量细胞钙的理想指标。这个Screen Quest Fluo-8 NW钙测定试剂盒提供了一种优化的检测方法，用于监测G蛋白偶联受体（GPCR）和钙通道。该测定可以以方便的96孔或384孔微量滴定板形式进行，并且易于适应自动化。百萤生物是AAT Bioquest的中国代理商，为您提供优质的Screen Quest Fluo-8 去除培养基钙检测试剂盒*适合于难检测细胞系*。 

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