货号：01-340-1B、C3240-0100

Product Description:
Living organisms differ considerably with respect to their ability to synthesize Amino Acids and the forms of nitrogen which they may utilize for such a purpose. The higher vertebrates do not possess the ability to synthesize all the common Amino Acids like some of the more versatile higher plants or microorganisms like E. coli that can. Humans and the albino rat can make only ten(10) of the twenty(20) AA's required for protein synthesis. These collectively are known as the Non-Essential Amino Acids (NEAA's), the remainder being the Essential Amino Acids (EAA's).

Amino acids are the building blocks (i.e. linear chains of amino acids) of proteins and proteins have a variety of functions in metabolism, may be precursors for the biosynthesis of other biological molecules, function with coenzymes, are critical to life and are therefore needed by every living organism. An amino acid is a molecule containing both amine and carboxyl functional groups. Proteins are chains of amino acids linked together by peptide bonds.

Chemically speaking, they are carboxylic acids which have an amine group attached to it. Next to water, protein makes up the largest portion of our body weight as it is contained in all the muscles, body organs, hair, nails and other body systems. There are more than 500 amino acids which occur in nature of which humans can produce 10 of 20 amino acids; the others must be supplied by the diet. Unlike fat and complex carbohydrates, the human body does not store excess amino acids as a reserve; they must be supplied in the diet every day. Concentrated supplements like MEM NEAA's 100X add nutrients that cells might not encounter thereby reducing the overall biosynthetic burden to cell cultures in vitro.

Amino acids are incorporated into proteins. At a minimum, basal medium must contain those essential amino acids (EAA's) that cannot be synthesized by the cells including L-Cysteine and L-Tyrosine at a rate to meet the metabolic requirements of the cells in culture. Individual requirements vary for the cell type being cultured. Some more specialized media often have non-essential amino acids (NEAA's) added to ensure that amino acids do not limit the maximum cell concentration attainable in order to compensate a particular cell type which is unable to manufacture them of if they leach rapidly into the medium.

As the selection of a nutrient medium or supplementation thereof is strongly influenced, among others, by many factors, of note are three major considerations:
◊ Cell Type
◊ Type of Culture(e.g., Clonal, Monolayer, Suspension)
◊ Degree of Chemical Definition

It is recommended to review the extensive literature concerning cell-culture media and its supplementation and the physiological parameters required for each specific cell-line as per their essential niche requirements.
Predominant Characteristics o f MEM Non-Essential Amino Acids Solution, 100 X Conc., includes:
§ Liquid 100X Concentrate
§ Stimulates Growth and Prolongs Cell Viability
§ Commonly Used In Cell Culture System Applications and Formulations
§ Relatively Long-Storage When Handled and Stored Properly Under Defined Conditions