pSTMV5
- TS131923
- 泰斯拓生物
- 浙江省宁波市
- 现货
- 按需定制
- 议价
- 2022-12-23 13:48:32
宁波泰斯拓生物技术有限责任公司
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产品名称：
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pSTMV5
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商品货号：
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TS131923
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Designations：
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pSTMV5
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GenBank Number：
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M25782
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Species：
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satellite tobacco mosaic tobamovirus (STMV)
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Depositors：
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G Kurath
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Applications：
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Linearize with NsiI and transcribe with T7 RNA polymerase to produce a sense strand.
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Vector：
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Construct size (kb): 4.300000190734863
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Insert：
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DNA: cDNA
Insert lengths(kb): 1.059000015258789
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Insert Size (kb)：
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1.059
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Media：
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ATCC Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin®
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Biosafety Level：
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1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
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Comments：
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Restriction digests of the clone give the following sizes (kb): XbaI/SphI--3.4, 1.15; SalI--4.4; BglII--4.4.
The insert contains the following restriction sites (bp from the 5 XbaI site): DraI--321; SalI--602; BglII--791. The primer-derived NsiI site is at nt 1059.
Clones pSTMV5 and pSTMV6 (TS131923, 45136) can be distinguished by RNase protection assays which give distinct patterns due to an A-C difference at nt 751 of the STMV sequence.
The RNA transcribed from this clone is infectious.
Contains a complete STMV genome representing the major genome type within the STMV type strain.
pSTMV5 is identical to the reported STMV type strain sequence except for a G to A difference at nt 494.
Linearize with NsiI and transcribe with T7 RNA polymerase to produce a sense strand.
Constructed using primers for cDNA synthesis that added NsiI and SphI sites at the 3 end and an XbaI site at the 5 end.
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Classification：
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Tobamovirus
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References：
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Kurath G, et al. Analysis of genetic heterogeneity within the type strain of satellite tobacco mosaic virus reveals several variants and a strong bias for G to A substitution mutations. Virology 189: 233-244, 1992. PubMed: 1604813
Mirkov TE, et al. Nucleotide sequence and translation of satellite tobacco mosaic virus RNA. Virology 170: 139-146, 1989. PubMed: 2718378
Gael Kurath, personal communication
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