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花生过敏原Ara h3检测试剂盒
Ara h 3 ELISA kit
  • EL-AH3
  • INDOOR BIOTECHNOLOGIES
  • 上海市
  • 一个月内
  • 1盒
  • 0
  • 2022-05-24 16:05:34

上海博升生物科技有限公司

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  • 英文名称
  • Ara h 3 ELISA kit
ELISA kit for measuring Arachis hypogaea allergen, Ara h 3.

Antibodies: 1E8/4G9

Sensitivity: 0.2ng/ml

Limit of Detection: 2.0ng/ml

Assay Performance:  
Contents:

Monoclonal antibody 1E8, Ara h 3 Allergen Standard, Biotinylated
antibody 4G9; sufficient reagents for 10 microtiter plates.

Storage: Store antibodies at 4°C; store ST-AH3 at -20°C upon arrival.

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20

(PBS-T)

Bovine serum albumin

Streptavidin-Peroxidase

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:  
Product Resources: EL-AH3 Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis

Monoclonal Antibody: 1E8

Vial: Red Cap; 100µl

Immunogen: Ara h 3

Isotype: Mouse IgG1

Specificity: Binds to species specific epitope present on Arachis

hypogaea
 allergen, Ara h 3.

Purification: Produced in vitro and purified by affinity chromatography using

Protein A. Single heavy and light chain bands on SDS-PAGE.

Concentration: 1.1 mg/ml in phosphate buffered saline, pH 7.4.  Based on A280 for

IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.


  Certificate of Analysis

Monoclonal Antibody: 4G9

Vial: Brown Cap; 100µl

Immunogen: Ara h 3

Isotype: Mouse IgG1

Specificity: Binds to species specific epitope present on Arachis

hypogaea
 allergen, Ara h 3.

Purification: Produced in vitro and purified by affinity chromatography using

Protein A. Single heavy and light chain bands on SDS-PAGE.

Biotinylation: Biotinylated and titrated for use in ELISA at 1/1000 dilution.

Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered,

preservative free.

  Certificate of Analysis

Allergen Standard: nAra h 3

Vial: White Cap; 400µl

Composition: Naturally purified Ara h 3 prepared in 1% BSA/50% glycerol/PBS,

pH 7.4.
Concentration: 1,250ng/ml Ara h 3

Calibration: The Ara h 3 concentration of the purified Ara h 3 was determined by

amino acid analysis.

Stability/Storage: Please store the standard at -20ºC (+/- 5°C).

 

References:

1.  Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6.

2.  Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24.

(For complete reference list see EL-AH3 Certificate of Analysis)

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