Purified from cat hair extract by multi-step column chromatography. Purity > 95% by SDS-PAGE.

Preservative and carrier-free in endotoxin free phosphate buffered saline, pH 7.4. Filtered through 0.22µm filter.

LoTox™ Natural Fel d 1 Certificate of Analysis

Focus on... Fel d 1

1. Chapman MD, Aalberse RC, Brown MJ, Platts-Mills TAE. Monoclonal antibodies to the major feline allergen, Fel d I: II. Single step affinity purification of Fel d I, N-terminal sequence analysis and development of a sensitive two-site immunoassay to assess Fel d I exposure. J Immunol 1988: 140:812-818.

2. Luczynska CM, Li Y, Chapman MD, Platts-Mills TAE. Airborne concentrations and particle size distribution of allergen derived from domestic cats (Felis domesticus). Amer Rev Respir Dis 1990: 141:361-367.

3. Morgenstern JP, Griffith IJ, Brauer AW, Rogers BL, Bond JF, Chapman MD, Kuo M. Amino acid sequence of Fel dI, the major allergen of the domestic cat: Protein sequence analysis and cDNA cloning. Proc Natl Acad Sci USA 1991;88:9690-4.

4. Kaiser L, Velickovic TC, Badia-Martinez D, Adedoyin J, Thunberg S, Hallen D, Berndt K, Gronlund H, Gafvelin G, van HM, Achour A. Structural Characterization of the Tetrameric form of the Major Cat Allergen Fel d 1. J Mol Biol, 2007;370:714-727.

5. Hulse KE, Reefer AJ, Engelhard VH, Satinover SM, Patrie JT, Chapman MD, Woodfolk JA. Targeting Fel d 1 to FcgammaRI induces a novel variation of the T(H)2 response in subjects with cat allergy. J Allergy Clin Immunol, 2008;121:756-762.