6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *Single isomer*
- 376-100 mg；376-1g
- 北京富百科
- 北京市
- 现货
- 100 mg
- 1g
- 4150 元
- 2023-03-20 16:27:39
北京富百科生物技术有限公司
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产品介绍
Fluorescent Dye Carboxylic Acids and Their Succinimidyl Esters
TAMRA® is the registered trademark and strictly forbidden to be used without authorization.
Succinimidyl esters are proven to be the best reagents for amine modifications because the amide bonds that are formed are essentially identical to, and as stable as the natural peptide bonds. These reagents are generally stable and show good reactivity and selectivity with aliphatic amines. There are few factors that need be considered when SE compounds are used for conjugation reaction:
1). Solvents:For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO).
2). Reaction pH:The labeling reactions of amines with succinimidyl esters are strongly pH dependent. Amine-reactive reagents react with non-protonated aliphatic amine groups, including the terminal amines of proteins and the e-amino groups of lysines. Thus amine acylation reactions are usually carried out above pH 7.5. Protein modifications by succinimidyl esters can typically be done at pH 7.5-8.5, whereas isothiocyanates may require a pH 9.0-10.0 for optimal conjugations.
3).Reaction Buffers:Buffers that contain free amines such as Tris and glycine and thiol compounds must be avoided when using an amine-reactive reagent. Ammonium salts (such as ammonium sulfate and ammonium acetate) that are widely used for protein precipitation must also be removed (such as viadinlysis) before performing dye conjugations.
4). Reaction Temperature:Most conjugations are done at room temperature. However, either elevated or reduced temperature may be required for a particular labeling reaction.
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester]
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Features and Biological Applications
6-TAMRA, SE is the other purified single isomer of 5(6)-TAMRA, SE. It is predominantly used for nucleotide labeling and DNA sequencing.
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References
1. Hsu TM, et al. (2001). Genotyping single-nucleotide polymorphisms by the invader assay with dual-color fluorescence polarization detection. Clin Chem47, 1373-7.
2. Sanders SJ (2000). Factor V Leiden genotyping using real-time fluorescent polymerase chain reaction. Mol Cell Probes14, 249-53.
3. Sanders Sevall, J., Factor v leiden genotyping using real-time fluorescent polymerase chain reaction.Mol Cell Probes2000, 14, 249-53.
4. Micka, K.A., et al., Twgdam validation of a nine-locus and a four-locus fluorescent str multiplex system.J Forensic Sci1999, 44, 1243-57.
1) 本公司所有产品仅限于专业人员用于生命科学研究，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅。
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产品参数
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Name
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6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *Single isomer*
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CAT#
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376-100 mg；376-1g
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CAS#
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150810-69-8
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Storage#
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−20°C and desiccated
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Shelf Life#
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12 months
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Ex(nm)#
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547
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Em(nm)#
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573
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MW#
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527.53
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Solvent#
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DMF or DMSO
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