Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OPA1 Polyclonal Antibody, Unconjugated(bs-11764R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
视神经萎缩相关蛋白1抗体
OPA1 Rabbit pAb
- bs-11764R
- 北京博奥森
- 北京市
- 现货
- 50ul
- 100ul
- 200ul
- 议价
- 2023-10-10 15:05:15
北京博奥森生物技术有限公司
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- 英文名称
- OPA1 Rabbit pAb
概述
产品编号
bs-11764R
产品分类
一抗
英文名称
OPA1 Rabbit pAb
中文名称
视神经萎缩相关蛋白1抗体
英文别名
Dynamin like 120 kDa protein; Dynamin like 120 kDa protein, mitochondrial; Dynamin-like 120 kDa protein; Dynamin-like 120 kDa protein, form S1; FLJ12460; Juvenile kjer type optic atrophy; Juvenile kjer-type optic atrophy; KIAA0567; KJER type; Large GTP binding protein; largeG; MGM1; Mitochondrial dynamin like 120 kDa protein; Mitochondrial dynamin like GTPase; NPG; NTG; OAK; OPA 1; OPA1; OPA1 gene; OPA1_HUMAN; Optic atrophy 1 (autosomal dominant); OPTIC ATROPHY 1; Optic atrophy 1 gene protein; Optic atrophy 1 homolog (human); Optic atrophy protein 1; Optic atrophy protein 1 homolog.
交叉反应
Rat（predicted：Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep）
抗体来源
Rabbit
免疫原
KLH conjugated synthetic peptide derived from human OPA1
亚型
IgG
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
111kDa
GeneID
4976
Swiss
O60313
浓度
1mg/ml
储存液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Subunit
Subcellular Location
Mitochondrion inner membrane. Mitochondrion intermembrane space.
Tissue Specificity
Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
Post-translational modifications
PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.
DISEASE
Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
Similarity
Belongs to the dynamin family.
Database links
Entrez Gene: 424900 Chicken
Entrez Gene: 4976 Human
Entrez Gene: 74143 Mouse
Omim: 605290 Human
SwissProt: O60313 Human
SwissProt: P58281 Mouse
Unigene: 594504 Human
Unigene: 274285 Mouse
Unigene: 9783 Rat
背景资料
OPA1 is a 120kDa protein belonging to the dynamin family. The OPA1 gene has been localized to 3q29. The gene is targeted to mitochondria and is involved in mitochondrial biogenesis. Defects in OPA1 are a cause of optic atrophy type 1. OPA1 is mostly expressed in retina but can also be expressed in brain, testis, heart and skeletal muscle.
应用
| 应用 | 推荐稀释比例 |
|---|---|
| ELISA | 1:5000-10000 |
| IF | 1:100-500 |
| ICC | 1:100-500 |
| IHC-F | 1:100-500 |
| IHC-P | 1:100-500 |
| WB | 1:500-2000 |
图片资料
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-OPA1 (bs-11764R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-OPA1 (bs-11764R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
