Monoclonal IgG antibodies were prepared by immunizing mice with preprorelaxin (Bioraytec Ltd., Zhuhai, China) using standard laboratory methods known in the art. Anti-preprorelaxin antibody expressing hybridomas were derived by fusing myeloma cells with splenocytes from the immunized mice. The antibodies produced by hybridoma clonal cell lines were initially screened for activity against relaxin-positive pregnant dog serum. Relaxin positivity of the serum was confirmed using the FAST est RELAXIN in vitro test (Diagnostik Megacor, Hbrbranz, Austria). The serum contains mature relaxin as preprorelaxin is not present in serum, and so the selected antibodies are reactive against relaxin and not only preprorelaxin.

通过与芬兰BELLYLABS公司合作，珠海佰瑞特生物科技有限公司开发出了犬松弛素单克隆抗体，并协助合作伙伴申请了国际专利。目前该抗体只可以在中国使用，国外的使用权已经出售给了芬兰公司。

专利全文连接

松弛素作为胰岛素超家族中的一种多肽激素，在生殖机能和很多非生殖生理过程中发挥重要的调控作用，而血中松弛素被看成是犬(Canis lupus)和部分其他动物妊娠的标志。

• 应用：可以用于层析、ELISA等诊断试剂的生产。
• 储存：存放于2-8℃，可保存至有效期
• 规格：mg
• 推荐配对：

配对：多株抗体与8F11配对无论是标记还是包被，综合性能都很出色。与进口胶体金试剂相比，灵敏度有大幅度的提升，可以更早检出早孕。

配对推荐：2R-0512/2R-0610, 2R-0610/2R-0512;  6R-0312/2R-087; 10R-0211/2R-087, 2R-087/10R-0211; 2R-087/6R-0312;