Application of serum flask in cell culture
The development of cell culture technology has been very mature, cell growth needs to meet the requirements of environment, temperature, osmotic pressure, PH value and other indicators, in a variety of synthetic medium to provide basic nutrients at the same time, but also according to different cells and different culture purposes to add serum and other substances to promote cell growth.
Therefore, serum flask has a wide range of application in the field of cell culture.
INTL KANG Media bottles are medium, serum and buffer in today's pharmaceutical, diagnostic and biotechnology marketsIdeal container for storing and transporting reagents.
Serum is a very complex mixture formed by removing fibrinogen from plasma, mainly including fetal bovine serum, calf serum, adult bovine serum, horse serum, sheep serum, chicken serum and rabbit serum, among which fetal bovine serum is the most commonly used.
Serum provides important substances such as extracellular matrix, growth factors and transferrin, and the proportion of serum added depends on different cells and research purposes.
10% ~ 20% serum can maintain the rapid growth and proliferation of cells, known as the growth medium; In order to maintain the slow growth or immortality of cells, 2% ~ 5% serum can be added, called maintenance culture.
Due to the particularity of serum, its storage temperature should be -5℃ to -20℃.
Serum bottle is generally made of transparent PET material processed by injection and blowing technology. This material has good heat aging resistance, embrittlement temperature is -70℃, and it still has certain toughness at -30℃, which meets the storage needs of serum.
And serum bottles will be sterilized, no heat source, no endotoxin, no DNA enzyme, RNA enzyme and other factors affecting cell growth.
Serum is indispensable for cell culture. With the continuous development of biological products, immunotherapy, scientific research institutes and other fields, the demand for serum will also increase, and serum bottles will also be more widely used in the field of cell culture.
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